Overview

CD25 MicroBeads II were developed for positive selection or depletion of CD25+ cells.

 

Detailed product information

Background information

CD25, the low-affinity interleukin-2 receptor alpha chain (IL-2Rα), is expressed on activated T and B cells and monocytes. CD25 is also present on a subset of non-activated CD4+ T cells, which has regulatory function.

 

The CD25 MicroBeads II recognize epitope A of the CD25 molecule.

 

For control staining of cells separated with CD25 MicroBeads II, it is recommended to use MACS® CD25 Antibodies, which bind to epitope B of the CD25 antigen.

 

For isolation of CD25+ cell subsets, the CD25 MicroBeads II can be combined with MACS® Cell Isolation Kits or MultiSort Kits. The CD4+CD25+ Regulatory T Cell Isolation Kit is recommended for most efficient separation of CD4+CD25+ regulatory T cells.

 

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.